Deriving Henderson-Hasselbalch Equation Step by Step. The Relationship between pH, pKa, Titration Curve and Buffer Action of a Weak Acid. The Significance. Derivation of the Henderson –Hasselbalch Equation for weak base. B + HOH. BH . +. + OH. – or. B. –. + HOH. BH + OH. –. Either one works, but you have to be. Henderson–Hasselbalch equation describes the derivation of pH as a measure of acidity using the acid dissociation constant (pKa.

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The most significant is the assumption that the concentration of the acid and its conjugate base at equilibrium will remain the same as the formal concentration. How Hydrogen Bond is formed in Water?

Then take the negative logarithm of both sides: In this example, we will look at the equilibrium of the weak acid, hydrofluoric acid. However, this approach is justifiable in many cases, it creates false conviction that the equation could be applied in all the situations which is not true Henderson-Hasselbalch equation is valid only when it contains equilibrium concentrations of acid and conjugate base.

Later, Karl Albert Hasselbalch re-expressed that formula in logarithmic terms, resulting in the Henderson—Hasselbalch equation. Leave a Reply Cancel reply You must be logged in to post a comment.

The more diluted the solution is, the more solution pH is dominated not by the presence of acetic acid and its conjugate base, but by the water auto dissociation.

Henderson-Hasselbalch equation is used mostly to calculate pH of solution which is obtained by mixing known amount of acid and conjugate base or neutralizing part of acid with strong base.

However, these are related by the equation: Taken eqkation, the following equation can be used to relate the pH of blood to the concentration of bicarbonate and the partial pressure of carbon dioxide: Articles needing expert attention with no hasselbapch or talk parameter Articles needing expert attention from February All articles needing expert attention Chemistry articles needing expert attention Articles needing additional references from February All articles needing additional references Articles with multiple maintenance issues All articles that may contain original research Articles that may contain eqaution research from February CS1: The equation is also useful for estimating the pH of a buffer solution and finding the equilibrium pH in acid-base reactions it is widely used to calculate the isoelectric point of proteins.


We have already stated that for a buffer the best buffering pH is at its pKa. Views Read Edit View history. Acid—base hendefson Equilibrium chemistry Equations Mathematics in medicine Enzyme kinetics. Please help improve it or discuss these issues on the talk page. Acetate is the conjugate base of acetic acid.


You might also like…. For the standard base equation: This is the best buffering pH of acetic acid. This article needs additional citations for verification.

Then, using the properties of logarithms:. From Wikipedia, the free encyclopedia.

Deriving the Henderson-Hasselbach Equation

Thus the equation 6 can be better stated as: Please subscribe to this blog! We also discuss the significance of Henderson-Hasselbalch equation.

However, the same equation will work perfectly regardless of the pKa value if you are asked to calculate ratio of acid to conjugate base in the solution with known pH. Our final step is to simplify our expression so that we are using pH and. Substitute the pH and pKa in the equation 4: The Henderson-Hasselbalch equation also describes the characteristic shape of the titration curve of any weak acid such as acetic acid, derivstion acid, or any amino acid.


For example, in the case of acetate buffer, the pKa is 4. The Henderson—Hasselbalch equation is derived from the acid dissociation constant equation by the following steps: Julian—Gregorian uncertainty Use dmy dates from September InLawrence Joseph Henderson wrote an equation describing the use of carbonic acid as a buffer solution.

The dissociation of water and relative water concentration itself is neglected as well. The Henderson—Hasselbalch is mainly equatio for calculating the pH or pKa of a solution containing known quantities of a weak acid and its conjugate base.

How do you derive the Henderson-Hasselbalch equation?

Why the pH of water is 7? Now a logarithm rule: The first step in deriving the Henderson-Hasselbach equation is to set up your equilibrium expression.

By determining the pH using either an equation or by pH meter we find the pH will be 1.

A little more manipulation and we can rearrange the part of the equation highlighted in blue. By using this site, you agree to the Terms of Use and Privacy Policy.

How do you derive the Henderson-Hasselbalch equation? | Socratic

For solutions of a weak base the equation below can be used. Hassslbalch dissociation constant Ka of the above reaction will be: In the present post, we will see the derivation of Henderson-Hasselbalch equation from the ionization reaction of a weak acid. It is worth noting here that 1 mM buffer solution has so low capacity, that it has very limited practical use.

The above equation is called the Henderson-Hasselbalch equation.